First in vitro directed biosynthesis of new compounds by a minimal type II polyketide synthase: evidence for the mechanism of chain length determination |
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| Authors: | Nicholson T P Winfield C Westcott J Crosby J Simpson T J Cox R J |
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| Institution: | School of Chemistry, University of Bristol, Cantock's Close, Bristol, UK BS8 1TS. |
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| Abstract: | The minimal actinorhodin polyketide synthase bearing two point mutations (KSbeta Q161A, ACP C17S) was chemically modified to carry novel C4 to C8 starter units on the ACP: on incubation with an excess of malonyl CoA new 16-carbon polyketides are made, supporting a measuring mechanism. |
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