Soluble and immobilized catalase |
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Authors: | P T Vasudevan D S Thakur |
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Institution: | (1) Department of Chemical Engineering, University of New Hampshire, 03824 Durham, NH;(2) Present address: Bristol Myers Squibb, Syracuse, NY |
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Abstract: | This article examines the effect of pressure on the steady-state kinetics and long-term deactivation of the enzyme catalase
supported on porous alumina. The reaction studied is the decomposition of hydrogen peroxide. The results of studies carried
out in a continuous stirred-tank reactor under isothermal conditions are presented and compared with results obtained for
soluble catalase. For soluble catalase, it is found that in the range of pressures studied, the oxygen flow rate increases
with increase in pressure up to a certain value and then decreases. Hydrogen peroxide concentration appears to have a strong
influence on pressure effects. With immobilized catalase, the pressure effects are not as prominent. Fluorescent microscopy
studies of the immobilized enzyme suggest that this is probably because of pore diffusional limitations. |
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Keywords: | Immobilized catalase inhibition pressure effects |
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