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Interaction of RuIII(EDTA) with cellular thiols and O2: biological implications thereof |
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| Authors: | Debabrata Chatterjee Namita Jaiswal Jagadeesh C Bose K Ram Balak Mahato Diptendu Bhattacharyya |
| Institution: | 1. Chemistry &2. Biomimetics Group, CSIR-Central Mechanical Engineering Research Institute, Durgapur, Indiadeb_cmeri@yahoo.com;4. Biomimetics Group, CSIR-Central Mechanical Engineering Research Institute, Durgapur, India;5. Department of Biotechnology, National Institute of Technology, Durgapur, India;6. Department of Chemistry, CSIR-Indian Institute of Chemical Biology, Kolkata, India |
| Abstract: | Reaction of RuIII(EDTA)(CyS)]2? (edta4? = ethylenediaminetetraacetate; CySH = cysteine) with molecular oxygen (O2) has been studied as a function of pH (4.0–8.0) and cysteine concentration (0.2–2.0 mM) at room temperature (25 °C). Biological activities of the Ru(EDTA)]/CySH/O2 system pertaining to cleavage of supercoiled plasmid DNA to its nicked open circular form has been explored in this work. Results are discussed in regard to the reaction of the ruthenium(III)-complex with molecular oxygen) and a working mechanism is proposed for the biological activities of the ruthenium(III)-complex in the presence of O2. |
| Keywords: | Ru(III)-EDTA complex Cysteine Redox reaction DNA cleavage |