Modification of polydivinylbenzene microspheres by a hydrobromination/click-chemistry protocol and their protein-adsorption properties |
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| Authors: | Durmaz Yasemin Yuksel Karagoz Bunyamin Bicak Niyazi Demirkol Dilek Odaci Yalcinkaya Esra Evrim Timur Suna Yagci Yusuf |
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| Institution: | Istanbul Technical University, Faculty of Science and Letters, Department of Chemistry, Maslak 34469 Istanbul, Turkey. |
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| Abstract: | Hydrophobic- and/or hydrophilic-polymer-grafted PDVB microspheres are synthesized by the combination of hydrobromination and click-chemistry processes. The modified-PDVB microspheres and the intermediates at various stages of synthesis are characterized using GPC, 1H NMR and FTIR spectroscopy and TGA analysis. Use of the microspheres as a support matrix for reversible protein immobilization via adsorption is investigated. The system parameters such as the adsorption conditions (i.e., enzyme concentration, medium pH) and desorption are studied and evaluated with regards to the biocatalytic activity and adsorption capacity. |
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| Keywords: | biomolecule immobilization click chemistry microspheres poly(divinylbenzene) surface modification |
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