CE‐ESI‐TOF/MS for human growth hormone analysis |
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| Authors: | Aline Staub Sylvain Giraud Martial Saugy Serge Rudaz Jean‐Luc Veuthey Julie Schappler |
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| Affiliation: | 1. School of Pharmaceutical Sciences, University of Geneva, University of Lausanne, Geneva, Switzerland;2. Swiss Centre for Applied Human Toxicology, University of Geneva, Geneva, Switzerland;3. Swiss Laboratory for Doping Analysis, Centre Universitaire Romand de Médecine Légale, Epalinges, Switzerland |
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| Abstract: | CE is a powerful analytical tool used to separate intact biomolecules such as proteins. The coupling of CE with TOF/MS produces a very promising method that can be used to detect and identify proteins in different matrices. This paper describes an efficient, rapid, and simple CE‐ESI‐TOF/MS procedure for the analysis of endogenous human growth hormone and recombinant human growth hormone without sample preparation. Operational factors were optimized using an experimental design, and the method was successfully applied to distinguish human growth hormone and recombinant human growth hormone in unknown samples. |
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| Keywords: | CE Human growth hormone MS TOF |
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