Chemische Vitamin D3-Bestimmung in Lebertran |
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Authors: | Syed Laik Ali |
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Affiliation: | 1.Zentrallaboratorium Deutscher Apotheker,Frankfurt |
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Abstract: | The different aspects of Vitamin D3 determination and the data reported in the literature are discussed. The spectrophotometric determination of Vitamin D3 in cod-liver oil is carried out after the alkaline saponification, extraction of unsaponified parts, precipitation of accompanying sterols and the column- and thin-layer chromatographic purification and separation of vitamin D3 on 40 cm plates from other vitamins. The dyestuff α-naphtholbenzein is suited well as standard substance for the better location and identification of vitamin D3 zone on the thin-layer plate. The results obtained from the chemical method were checked through the simultaneous biological determination. |
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