Resolution of an intense sweetener mixture by use of a flow injection sensor with on-line solid-phase extraction |
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Authors: | L F Capitán-Vallvey M C Valencia E Arana Nicolás J F García-Jiménez |
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Institution: | (1) Department of Analytical Chemistry, Faculty of Sciences, Campus Fuentenueva, University of Granada, 18071 Granada, Spain |
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Abstract: | An integrated solid-phase spectrophotometry–FIA method is proposed for simultaneous determination of the mixture of saccharin
(1,2-benzisothiazol-3(2H)-one-1,1-dioxide; E-954) (SA) and aspartame (N-l-α-aspartyl-l-phenylalanine-1-methyl ester; E-951) (AS). The procedure is based on on-line preconcentration of AS on a C18 silica gel minicolumn and separation from SA, followed by measurement, at λ=210 nm, of the absorbance of SA which is transiently retained on the adsorbent Sephadex G-25 placed in the flow-through cell
of a monochannel FIA setup using pH 3.0 orthophosphoric acid–dihydrogen phosphate buffer, 3.75×10–3 mol L−1, as carrier. Subsequent desorption of AS with methanol enables its determination at λ=205 nm. With a sampling frequency of 10 h−1, the applicable concentration range, the detection limit, and the relative standard deviation were from 1.0 to 200.0 μg mL−1, 0.30 μg mL−1, and 1.0% (80 μg mL−1, n=10), respectively, for SA and from 10.0 to 200.0 μg mL−1, 1.4 μg mL−1, and 1.6% (100 μg mL−1, n=10) for AS. The method was used to determine the amounts of aspartame and saccharin in sweets and drinks. Recovery was always
between 99 and 101%. The method enabled satisfactory determination of blends of SA and AS in low-calorie and dietary products
and the results were compared with those from an HPLC reference method. |
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Keywords: | Biparameter sensor Aspartame and saccharin mixture analysis Flow-through optical sensor On-line solid phase extraction Sweets and drink products |
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