Metabolism Studies of Polycyclic Mutagens and Carcinogens Using Liquid Chromatography with Ultraviolet and Electrochemical Detection

Abstract

HPLC ultraviolet (UV) and electrochemical (EC) detectors connected in series were used to classify the metabolites of naphthalene, anthracene, acridine, and phenanthridine into phenolic and nonphenolic categories. This method of classification is based on comparison of the relatively high response of the EC detector to electroactive species such as phenols to the more nonselective response of the UV detector. The method is generally useful for many biologically active substances.