Protein Purification on a New Preparative Ion Exchanger

Abstract

The ACCELL chromatographic media is a new packing designed specifically for the isolation and purification of proteins. The anionic and cationic functional groups are bonded to an encapsulated 40μ silica particle which can be readily packed into any size column. These columns can be operated on both high performance and medium performance liquid chromatographic equipment. The optimization of the separation conditions on the anion exchange media for the preparative purification of a monoclonal antibody (Mab) from ascites will be discussed as well as a multi-step purification of the enzyme prostatic acid phosphatase (PAP) from seminal fluids on the anion and cation exchangers.