Comparison of Methods for Determination of the RM Values of Substances in Thin - Layer Chromatography by Using Binary Mobile Phase

Abstract

Methods of calculating the function R_M= f(?₁) of some aromatic substances from the data of experimental and theoretically calculated parameters have been compared. It has been shown that there is a possibility to calculate this function with high accuracy on the basis of the measured partition coefficients of the substances chromatographed in partition systems consisting of components of the mobile phase as well as parameters obtained theoretically. Thus obtained results were also compared with the functions R_{M 1.2} = f (?₁) obtained experimentally and a high agreement was reached. This in turn contributes to optimization of systems of thin-layer chromatography.