Measurement of Cellulolytic Activity by Low Pressure Liquid Chromatography

Abstract

The application of aqueous, low pressure liquid chromatography to the assay of cellulolytic enzyme activity is discussed. The advantages of this method are speed of analysis (less than 20 min.), small sample size (20 μl), good resolution, and a tolerance of the system to the presence of extraneous salts and proteins. Examples showing the use of this tool are given.