| 荧光法研究芦丁-Cu2+/CO2+-BSA的相互作用 |
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| 引用本文： | 乐薇,吴小霞.荧光法研究芦丁-Cu2+/CO2+-BSA的相互作用[J].分子科学学报,2011,27(1):66-70. |
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| 作者姓名： | 乐薇 吴小霞 |
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| 作者单位： | 中南民族大学工商学院环境与生命科学系; |
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| 摘 要： | 采用荧光光谱、紫外-可见光谱技术研究了芦丁与牛血清白蛋白(BSA)在金属离子Cu2+/CO2+共存时的相互作用.研究结果表明:有或无金属离子存在时,芦丁对BSA的荧光猝灭作用都是由于生成复合物而引起的静态猝灭;金属离子的存在使芦丁与BSA的表观结合常数KLB增大,且Co使KL)增大得更明显.由热力学参数确定芦丁和BSA...
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| 关 键 词： | 芦丁 牛血清白蛋白 Co2+ Cu2+ 荧光光谱法 |
Study on the interaction of Rutin-Cu2+/CO2+-BSA by fluorescence |
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| YUE Wei,WU Xiao-xia.Study on the interaction of Rutin-Cu2+/CO2+-BSA by fluorescence[J].Journal of Molecular Science,2011,27(1):66-70. |
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| Authors: | YUE Wei WU Xiao-xia |
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| Institution: | YUE Wei,WU Xiao-xia(Department of Environment and Life Science,Engineering and Commerce College of South-Central University for Nationalities,Wuhan 430065,China) |
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| Abstract: | The effects of Cu2+ or Co2+ on the interaction between the rutin and bovine serum albumin(BSA) were investigated by means of UV-Vis and fluorescence spectroscopy.The results show that the fluorescence quenching of BSA by rutin is due to the formation of rutin-BSA complex and quenching mechanism is mainly static quenching.It was discovered that the apparent association constant(KLB) increases in the presence of Cu2+ or Co2+,and the effect of Co2+ is stronger.According to the thermodynamic parameters,the main... |
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| Keywords: | Rutin bovine serum albumin Co2 Cu2 fluorescence spectrometry |
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