| 重离子对人胃癌细胞DNA错配修复基因MSH2 表达的影响 |
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| 引用本文： | 缪国英,张红,卢启明,李鸿岩,狄翠霞,郭逸潇. 重离子对人胃癌细胞DNA错配修复基因MSH2 表达的影响[J]. 原子核物理评论, 2015, 32(1): 110-114. DOI: 10.11804/NuclPhysRev.32.01.110 |
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| 作者姓名： | 缪国英 张红 卢启明 李鸿岩 狄翠霞 郭逸潇 |
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| 作者单位： | 中国科学院近代物理研究所,兰州 730000; |
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| 基金项目： | 国家重点基础研究发展计划(973计划)项目(2010CB834202);国家自然科学基金资助项目(10835011,10675151);甘肃省重大科技专项项目(0702NKDA045,0806RJYA020);中国科学院西部之光人才培养计划项目(XB106012);甘肃省科技支撑计划资助(1104FKCA155);甘肃省自然科学基金资助(096RJZA030)~~ |
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| 摘 要： | 采用高传能线密度(LET) 重离子辐照人胃癌SGC7901 细胞，应用流式细胞技术、蛋白质印迹法(Western blot) 及反转录聚合酶链式反应(RT-PCR) 观察重离子诱导人胃癌SGC7901 细胞周期、凋亡和MSH2 表达状况。结果表明: 与对照组相比，SGC7901 细胞在辐射后72 h G2/M 期所占细胞比率(33.26±0.08) 和凋亡率(24.16±0.64) 均达到峰值，且呈时间依赖性增加；经重离子照射后，DNA错配修复基因MSH2 mRNA 和蛋白表达水平在6 h 最高。结果提示:重离子在体外诱导SGC7901 细胞周期阻滞和凋亡，且具有显著的时间依赖性效应；重离子在一定剂量和时间下，诱导了SGC7901 细胞MSH2 基因表达。DNA错配修复基因MSH2 可能参与了重离子辐照诱导胃癌细胞DNA损伤的修复应答。Human gastric cancer cell SGC7901 were irradiated with high linear energy transfer (LET) carbon ion. Apoptotic cells after irradiation were analyzed by flow cytometry and expression of MSH2 genes in the irradiated cells was detected by western blot and RT-PCR assay. Compared with the control group, we found that the number of G2/M (33.26±0.08) or apoptosis (24.16±0.64) of SGC7901 cells reached a maximum after irradiation at 72 h in a dose dependent manner. And heavy ion irradiation efficiently up-regulated the expression of MSH2 gene at 4.0 Gy after being irradiated 6 h. These results imply that heavy ion beam could induce cell apoptosis and cell cycle arrest in time-dependent manners. Furthermore, expression of MSH2 genes activated by carbon ion irradiation suggests that DNA mismatch repair gene MSH2 might be involved in DNA repair pathways.
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| 关 键 词： | 细胞凋亡 重离子束 MSH2 基因表达 |
| 收稿时间： | 1900-01-01 |
Effects of Heavy Ion Irradiation on MSH2 Expression in Human Gastric Cancer Cell SGC7901 |
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| MIAO Guoying;ZHANG Hong;LU Qiming;LI Hongyan;DI Cuixia;GUO Yixiao. Effects of Heavy Ion Irradiation on MSH2 Expression in Human Gastric Cancer Cell SGC7901[J]. Nuclear Physics Review, 2015, 32(1): 110-114. DOI: 10.11804/NuclPhysRev.32.01.110 |
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| Authors: | MIAO Guoying ZHANG Hong LU Qiming LI Hongyan DI Cuixia GUO Yixiao |
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| Affiliation: | Institute of Modern Physics, Chinese Academy of Sciences, Lanzhou 730000, China;Key Laboratory of Heavy Ion Radiation Biology and Medicine of Chinese Academy ofSciences, Lanzhou 730000, China;Key Laboratory of Heavy Ion Radiation Medicine of Gansu Province, Lanzhou 730000, China;University of Chinese Academy of Sciences, Beijing 100049, China; |
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| Abstract: | Human gastric cancer cell SGC7901 were irradiated with high linear energy transfer (LET) carbon ion. Apoptotic cells after irradiation were analyzed by flow cytometry and expression of MSH2 genes in the irradiated cells was detected by western blot and RT-PCR assay. Compared with the control group, we found that the number of G2/M (33.26±0.08) or apoptosis (24.16±0.64) of SGC7901 cells reached a maximum after irradiation at 72 h in a dose dependent manner. And heavy ion irradiation efficiently up-regulated the expression of MSH2 gene at 4.0 Gy after being irradiated 6 h. These results imply that heavy ion beam could induce cell apoptosis and cell cycle arrest in time-dependent manners. Furthermore, expression of MSH2 genes activated by carbon ion irradiation suggests that DNA mismatch repair gene MSH2 might be involved in DNA repair pathways. |
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