DNA‐Mediated Proximity‐Based Assembly Circuit for Actuation of Biochemical Reactions |
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Authors: | Sung Won Oh Adriana Pereira Ting Zhang Tianran Li Ariel Lane Dr Jinglin Fu |
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Institution: | 1. Center for Computational and Integrative Biology, Rutgers University—Camden, Camden, NJ, USA;2. Department of Chemistry, Rutgers University—Camden, Camden, NJ, USA |
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Abstract: | Smart nanodevices that integrate molecular recognition and signal production hold great promise for the point‐of‐care (POC) diagnostic applications. Herein, the development of a DNA‐mediated proximity assembly of biochemical reactions, which was capable of sensing various bio‐targets and reporting easy‐to‐read signals is reported. The circuit was composed of a DNA hairpin‐locked catalytic cofactor with inhibited activity. Specific molecular inputs can trigger a conformational switch of the DNA locks through the mechanisms of toehold displacement and aptamer switching, exposing an active cofactor. The subsequent assembly of an enzyme/cofactor pair actuated a reaction to produce colorimetric or fluorescence signals for detecting target molecules. The developed system could be potentially applied to smart biosensing in molecular diagnostics and POC tests. |
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Keywords: | biosensors DNA nanotechnology DNA assembly enzyme actuation logic gates |
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