| 青霉素酰化酶在含铁MCM-41介孔分子筛上的固定化研究 |
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| 引用本文： | 薛屏,卢冠忠,郭杨龙,王筠松,吴东方.青霉素酰化酶在含铁MCM-41介孔分子筛上的固定化研究[J].化学通报,2003,66(10):681-683. |
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| 作者姓名： | 薛屏 卢冠忠 郭杨龙 王筠松 吴东方 |
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| 作者单位： | 华东理工大学工业催化研究所，华东理工大学工业催化研究所，华东理工大学工业催化研究所，华东理工大学工业催化研究所，华东理工大学工业催化研究所 上海200237，宁夏大学化学与化工学院宁夏银川750021，上海200237，上海200237，上海200237，上海200237 |
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| 摘 要： | 制备了具有长程有序结构、孔径分布狭窄的含铁MCM-41介孔分子筛，利用直接法和共价结合法将青霉素酰化酶固定在分子筛表面。结果表明，两种方法制备的固定化酶对青霉素G水解反应的表观活性分别为782U/g和256U／g；经6次连续操作使用，二者保持初始活性的49．4％和81．2％，后者的操作稳定性好于前者。共价结合法制备的固定化酶活性较低，是由于Fe—MCM-41表面修饰后比表面积和孔径明显减小所致。
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| 关 键 词： | 青霉素酰化酶 含铁MCM-41介孔分子筛 固定化 载体 |
Study on Immobilization of Penicillin Acylase on Mesoporous Fe-MCM-41 Molecular Sieve |
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| Xue Ping a,b,Lu Guangzhong a,Guo Yanglong a,Wang Yunsong a,Wu Dongfang a.Study on Immobilization of Penicillin Acylase on Mesoporous Fe-MCM-41 Molecular Sieve[J].Chemistry,2003,66(10):681-683. |
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| Authors: | Xue Ping a b Lu Guangzhong a Guo Yanglong a Wang Yunsong a Wu Dongfang a |
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| Institution: | Xue Ping a,b,Lu Guangzhong a*,Guo Yanglong a,Wang Yunsong a,Wu Dongfang a |
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| Abstract: | The mesoporous MCM-41 molecular sieve containing iron having well ordered long\|range structure and narrow pore size distribution was synthesized. The apparent activity of penicillin acylase immobilized on Fe-MCM-41 through direct immobilization or covalent coupling was 782U/g and 268U/g, respectively. The former remained about 49.4% of its initial activity, and the latter remained 81.2% of its initial activity after used in the consecutive hydrolysis of penicillin G for 6 times. The enzyme immobilized by covalent coupling had lower initial activity due to the decrease of the pore diameter and the specific surthce area after Fe-MCM-41 was modified. |
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| Keywords: | Fe-MCM-41 Support Penicillin acylase Immobilization method |
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