Radioimmunoassay of human chorionic gonodotropin |
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Authors: | Ms M A Majali K B Shah R S Mani |
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Institution: | (1) Radiopharmaceuticals Section, Bhabha Atomic Research Centre, Trombay, Bombay, (India) |
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Abstract: | A rapid and sensitive radioimmunoassay has been developed for the measurement of Human Chorionic Gonadotropin (HCG) in serum.
Human chorionic gonadotropin has been labelled with125I to attain a specific activity between 80–120 μCi/μg. Aqueous dioxane (74 vol. %) has been employed to separate the bound
and freehormone in the radioimmunoassay. The sensitivity of this technique has been found to be ∼1.5 mIU/ml of HCG. The intra
assay variation has been found to be less than 5% and inter assay variation has been found to be less than 12%. |
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