| 微流控进样阿达玛变换显微荧光成像单细胞分析 |
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| 引用本文： | 李涛,唐宏武,周锦松,陈观铨. 微流控进样阿达玛变换显微荧光成像单细胞分析[J]. 分析化学, 2004, 32(5): 579-581 |
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| 作者姓名： | 李涛 唐宏武 周锦松 陈观铨 |
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| 作者单位： | 武汉大学化学与分子科学学院测试中心,武汉,430072;武汉大学化学与分子科学学院测试中心,武汉,430072;武汉大学化学与分子科学学院测试中心,武汉,430072;武汉大学化学与分子科学学院测试中心,武汉,430072 |
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| 基金项目： | 国家自然科学基金资助项目 (No .2 0 0 75 0 19) |
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| 摘 要： | 将微流控芯片用于单细胞进样，以自制阿达玛变换显微荧光成像分析系统进行成像检测，讨论了影响单细胞进样和荧光成像的主要因素，并对花粉细胞DNA含量进行定量分析。结果表明：微流控进样技术与HT显微成像技术相结合，可有效可靠地应用于单细胞分析中，所测定的三个玉帘花粉的DNA含量分别为124．4、123．9和62．9pg。
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| 关 键 词： | 阿达玛变换 微流控芯片 荧光成像 单细胞分析 |
Microfluidic Sampling and Hadamard Transform Microscopic Fluorescence Image Analysis for Single Cells |
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| Li Tao,Tang Hongwu ,Zhou Jinsong,Chen Guanquan. Microfluidic Sampling and Hadamard Transform Microscopic Fluorescence Image Analysis for Single Cells[J]. Chinese Journal of Analytical Chemistry, 2004, 32(5): 579-581 |
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| Authors: | Li Tao Tang Hongwu Zhou Jinsong Chen Guanquan |
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| Affiliation: | Li Tao,Tang Hongwu *,Zhou Jinsong,Chen Guanquan |
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| Abstract: | A microchip was applied to the sampling of single cell, the domestic-made Hadamard transform (HT) microscopic fluorescence imaging analysis system was used for imaging detection. The factors that affect single-cell sampling and imaging were discussed, and the deoxyribonucleic acid contents of the single pollens were measured quantitatively. The result shows that the system of microchip sampling combining with the HT microscopic imaging technique can be applied effectively and reliably for single-cell analysis, and the DNA contents in three measured Zephyranthes candida (Lindl.) Herb. pollens are 124.4, 123.9 and 62.9 pg, repectively . |
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| Keywords: | Hadamard transform microchip fluorescence imaging single-cell analysis |
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