| Using Toad Oocytes as an Ultrasensitive System for Estimating the Biological Activity of Trichosanthin |
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| 作者姓名： | 熊用周 朱光 左嘉客 王亚辉 |
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| 作者单位： | Shanghai Institute of Cell Biology，Academia Sinica，Shanghai 200031，PRC，Shanghai Institute of Cell Biology，Academia Sinica，Shanghai 200031，PRC，Shanghai Institute of Cell Biology，Academia Sinica，Shanghai 200031，PRC，Shanghai Institute of Cell Biology，Academia Sinica，Shanghai 200031，PRC |
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| 基金项目： | Key project supported by Academia Sinica. |
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| 摘 要： | A sensitive bioassay for estimating the activity of Trichosanthin was developed by using intraoocyte microinjection as the technique and germinal vescicle breakdown as a marker. The GVBD inhibitory activity assay was million-fold more sensitive than the routinely used abortifacient activity assay. The detectable amount of Trichosanthin ranged from 0.5 to 100 pg. Since the least detectable dose was 0.5 pg, it made the assay suitable to analyse the microquantity of pure Trichosanthin used for studying the structure and function of the drug.
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Using Toad Oocytes as an Ultrasensitive System for Estimating the Biological Activity of Trichosanthin |
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| XIONG Yong-Zhou ZHU Guang ZUO Jia-Ke and WANG Ya-Hui.Using Toad Oocytes as an Ultrasensitive System for Estimating the Biological Activity of Trichosanthin[J].Science in China(Chemistry),1993(4). |
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| Authors: | XIONG Yong-Zhou ZHU Guang ZUO Jia-Ke and WANG Ya-Hui |
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| Abstract: | A sensitive bioassay for estimating the activity of Trichosanthin was developed by using intraoocyte microinjection as the technique and germinal vescicle breakdown as a marker. The GVBD inhibitory activity assay was million-fold more sensitive than the routinely used abortifacient activity assay. The detectable amount of Trichosanthin ranged from 0.5 to 100 pg. Since the least detectable dose was 0.5 pg, it made the assay suitable to analyse the microquantity of pure Trichosanthin used for studying the structure and function of the drug. |
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| Keywords: | Trichosanthin toad oocyte maturation promoting factor germinal vesicle breakdown |
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