| 高效液相色谱-蒸发光散射检测法测定番茄中的番茄皂苷A |
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| 引用本文： | 卢凤来,刘金磊,陈月圆,吴建璋,李典鹏.高效液相色谱-蒸发光散射检测法测定番茄中的番茄皂苷A[J].色谱,2010,28(9):908-911. |
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| 作者姓名： | 卢凤来 刘金磊 陈月圆 吴建璋 李典鹏 |
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| 作者单位： | 广西壮族自治区、中国科学院广西植物研究所, 广西 桂林 541006 |
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| 基金项目： | 广西科学基金应用基础研究专项项目，桂林市科学研究与技术开发项目 |
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| 摘 要： | 建立了测定番茄原料中的番茄皂苷A含量的高效液相色谱-蒸发光散射检测法(HPLC-ELSD)。将实验条件优化后,运用该方法分离番茄皂苷A。结果表明,该方法在番茄皂苷A的质量为0.61～3.05 mg范围内具有良好的线性相关性(r=0.9995),平均回收率为97.9%～104.8%,相对标准偏差(RSD)≤4.14%(n=5)。该方法快速、准确,样品处理简单,可用于番茄原料及其提取物中番茄皂苷A的含量测定与质量控制。
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| 关 键 词： | 高效液相色谱法 蒸发光散射检测 番茄皂苷A 番茄 |
| 收稿时间： | 2010-06-28 |
Determination of esculeoside A in Lycopersicon esculentum MILL by high performance liquid chromatography with evaporative light scattering detection |
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| LU Fenglai,LIU Jinlei,CHEN Yueyuan,WU Jianzhang,LI Dianpeng.Determination of esculeoside A in Lycopersicon esculentum MILL by high performance liquid chromatography with evaporative light scattering detection[J].Chinese Journal of Chromatography,2010,28(9):908-911. |
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| Authors: | LU Fenglai LIU Jinlei CHEN Yueyuan WU Jianzhang LI Dianpeng |
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| Institution: | Guangxi Institute of Botany, Chinese Academy of Sciences, Guilin 541006, China |
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| Abstract: | A method for the determination of esculeoside A in Lycopersicon esculentum MILL by high performance liquid chromatography (HPLC) with evaporative light scattering detection (ELSD) was established. The results showed that esculeoside A had good linearity in the range of 0.61~3.05 mg with the correlation coefficient of 0.9995. The average recoveries were 97.9%~104.8% with the relative standard deviations (RSD) ≤4.14% (n=5). The method is simple, sensitive and suitable for the analysis of esculeoside A and quality control for the raw material and extract of Lycopersicon esculentum MILL. |
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| Keywords: | high performance liquid chromatography (HPLC) evaporative light scattering detection (ELSD) esculeoside A Lycopersicon esculentum MILL |
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