Synthesis of fluorescently labeled UDP-GlcNAc analogues and their evaluation as chitin synthase substrates |
| |
Authors: | Yeager Adam R Finney Nathaniel S |
| |
Institution: | Department of Chemistry and Biochemistry, University of California, San Diego, 9500 Gilman Dr., La Jolla, California 92093, USA. |
| |
Abstract: | structure: see text] Chitin synthase (CS) polymerizes UDP-GlcNAc to form chitin (poly-beta(1,4)-GlcNAc), a key component of fungal cell wall biosynthesis. Little is known about the substrate specificity of chitin synthase or the scope of substrate modification the enzyme will tolerate. Following a previous report suggesting that 6-O-dansyl GlcNAc is biosynthetically incorporated into chitin, we became interested in developing an assay for CS activity based on incorporation of a fluorescent substrate. We describe the synthesis of two fluorescent UDP-GlcNAc analogues and their evaluation as chitin synthase substrates. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |
|