Microcalorimetry as a possible tool for phenanthrene toxicity evaluation to eukaryotic cells |
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Authors: | Katarzyna Lisowska Jerzy D?ugoński |
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Institution: | a Department of Industrial Microbiology and Biotechnology, University of ?ód?, Banacha 12/16, 90-237 ?ód?, Poland b Department of Physical Chemistry, University of ?ód?, Pomorska 18, 91-416 ?ód?, Poland |
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Abstract: | Microcalorimetry and measurement of culture turbidity using a Bioscreen C Analyzer System were applied to study the toxic effect of phenanthrene on Cunninghamella elegans IM 1785/21Gp spore germination. The results of C. elegans spore incubation in Bioscreen C microbiology reader showed the inhibition of spore germination by 70% (with 25 mg l−1 of phenanthrene) and total inhibition of the fungus growth with a higher content of the xenobiotic (50-100 mg l−1). The microcalorimetric technique showed to be useful for the estimation of metabolic activity of C. elegans spores in growth medium up to xenobiotic concentrations of 90 mg l−1. These data corresponded with the microscopic observations. The obtained results showed that the microcalorimetry method could be a valuable supplement in the study on the mechanism of PAHs detoxification by fungi. |
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Keywords: | Cunninghamella elegans Microcalorimetry Phenanthrene Fungus Growth |
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