探针3-(2-氰基乙基)胞嘧啶同步荧光法测定血清中蛋白质

在模拟人体生理条件下，基于3-(2-氰基乙基)胞嘧啶(CECT)与人血清白蛋白(HSA)和牛血清白蛋白(BSA)之间的相互作用，以CECT为分子光谱探针研究了CECT-蛋白质体系的同步荧光光谱特征。同步荧光光谱特征及强度与Δλ值、反应介质、反应温度等因素有关。在此基础上，建立了以CECT为分子光谱探针定量测定血清样品中蛋白质含量的新方法。在最佳实验条件下，CECT-HSA和CECT-BSA体系的同步荧光强度分别在0～441.4和0～351.0 μg·mL-1的浓度范围内与蛋白质浓度呈现良好的线性关系, 检测限分别为0.023和0.035 μg·mL-1,相对标准偏差(RSD)1.2%～3.3%, 加标回收率为97.2%～100.4%。该方法具有简单、快速、灵敏度较高、线性范围宽、精密度和回收率较好等优点。该法可直接用于血清样品中蛋白质总量的测定，结果令人满意。

Determination of the Proteins in Serum Albumins by Synchronous Fluorescence Technique with 3-(2-Cyanoethyl)Cytosine as a Probe

Under simulative biological conditions and upon the interactions between 3-(2-cyanoethyl) cytosine (CECT) and human serum albumin (HSA) or bovine serum albumin (BSA), the authors studied the characteristics of synchronous fluorescence spectra of CECT-protein system with CECT as a molecular spectral probe. The spectral characteristics and intensity of synchronous fluorescence were related to the value of deltalambda, reaction medium, reaction temperature and so on. On the basis of these, the new method for the determination of proteins in serum albumin was developed with CECT as a molecular spectral probe. Under the optimum experimental conditions, the synchronous fluorescence intensities of CECT-HSA and CECT-BSA systems were in good proportion to the concentrations of HSA and BSA in the ranges of 0-441.4 and 0-351.0 microg x mL(-1), respectively. The detection limits were 0.023 and 0.035 microg x mL(-1), respectively. Relative standard deviations located between 12% and 3.3%, and the addition standard recovery was 97.2%-100.4% for all elements. The results suggested that the method features easy of implementation, rapidity, high sensitivity, broad linear range, and better RSD and recovery etc., and was employed directly to determine the total proteins in serum albumin samples with satisfactory results.