High‐performance liquid chromatographic separations of stereoisomers of chiral basic agrochemicals with polysaccharide‐based chiral columns and polar organic mobile phases |
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| Authors: | Iza Matarashvili Iamze Shvangiradze Lali Chankvetadze Shota Sidamonidze Nino Takaishvili Tivadar Farkas Bezhan Chankvetadze |
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| Institution: | 1. Institute of Physical and Analytical Chemistry, School of Exact and Natural Sciences, Tbilisi State University, Georgia;2. Department of Ecology and Environmental Protection, Faculty of Informatics, Mathematics and Natural Sciences, Georgian University of St. Andrew the Apostle, Georgia;3. Phenomenex Inc, Torrance, USA |
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| Abstract: | The separation of the stereoisomers of 23 chiral basic agrochemicals was studied on six different polysaccharide‐based chiral columns in high‐performance liquid chromatography with various polar organic mobile phases. Along with the successful separation of analyte stereoisomers, emphasis was placed on the effect of the chiral selector and mobile phase composition on the elution order of stereoisomers. The interesting phenomenon of reversal of enantiomer/stereoisomer elution order function of the polysaccharide backbone (cellulose or amylose), type of derivative (carbamate or benzoate), nature, and position of the substituent(s) in the phenylcarbamate moiety (methyl or chloro) and the nature of the mobile phase was observed. For several of the analytes containing two chiral centers all four stereoisomers were resolved with at least one chiral selector/mobile phase combination. |
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| Keywords: | Chiral agrochemicals Enantiomer elution order High‐performance liquid chromatography Stereoisomer elution order |
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