| 亚油酸酰化胰岛素的制备及飞行质谱分析 |
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| 引用本文： | 黄涛,黄开勋.亚油酸酰化胰岛素的制备及飞行质谱分析[J].化学研究与应用,2006,18(7):834-836. |
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| 作者姓名： | 黄涛 黄开勋 |
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| 作者单位： | 1. 中南民族大学化学与材料学院分析化学国家民委重点实验室,湖北,武汉,430074
2. 华中科技大学化学系,湖北,武汉,430073 |
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| 基金项目： | 湖北省教育厅科研基金资助项目(2003A007) |
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| 摘 要： | 胰岛素侧链氨基的酰化是胰岛素化学修饰较常用的方法。天然胰岛素分子中有三个裸露的氨基,即G lyA1、PheB1的α-氨基和LysB29的ε-氨基,都可作为胰岛素化学修饰的位点。酰化试剂通常经由N-羟基琥珀酰亚胺酯对胰岛素进行修饰。N-羟基琥珀酰亚胺酯可由羧酸与N-羟基琥珀酰亚胺(HOS
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| 关 键 词： | 亚油酸 苯并三唑 亚油酰苯并三唑 胰岛素 亚油酰胰岛素 |
| 文章编号： | 1004-1656（2006）07-0834-03 |
| 收稿时间： | 2005-06-08 |
| 修稿时间： | 2005-10-02 |
Preparation and MALDI-TOF-MS analysis of the acylated insulin with linoleic acid |
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| HUANG Tao,HUANG Kai-xun.Preparation and MALDI-TOF-MS analysis of the acylated insulin with linoleic acid[J].Chemical Research and Application,2006,18(7):834-836. |
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| Authors: | HUANG Tao HUANG Kai-xun |
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| Institution: | 1. Key Laboratory for Analytical Chemistry of the State Ethnic Affairs Commission, College of Chemistry and Material Science, South -Central University for Nationalities, Wuhan 430074, China; 2. Department of Chemistry, Huazhong University of Science and Technology, Wuhan 430073, China |
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| Abstract: | Linoleoyl benzotriazole was synthesized from linoleic acid and benzotriazole by means of dicyclohexylcarbodiimide coupling method and characterized by FT-IR and ~(1)H NMR.Linoleoyl insulin was prepared by chemical modification of insulin with linoleoyl benzotriazole without using any specific protecting group.MALDI-TOF-MS analysis showed that the modification of insulin was successful and the monoacylated insulin was the major product.A little diacylated insulin was observed and no triacylated insulin was detected. |
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| Keywords: | linoleic acid benzotriazole linoleoyl benzotriazole insulin linoleoyl insulin |
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