Immobilization in biotechnology and biorecognition: from macro- to nanoscale systems |
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| Authors: | Marek Bu?ko Danica Mislovi?ová Jozef Nahálka Alica Vikartovská Jana ?ef?ovi?ová Jaroslav Katrlík Ján Tká? Peter Gemeiner Igor Lacík Vladimír ?tefuca Milan Polakovi? Michal Rosenberg Martin Rebro? Daniela ?mogrovi?ová Juraj ?vitel |
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| Institution: | 1.Institute of Chemistry, Centre for Glycomics,Slovak Academy of Sciences,Bratislava,Slovakia;2.Polymer Institute,Slovak Academy of Sciences,Bratislava,Slovakia;3.Axxence Slovakia s.r.o.,Bratislava,Slovakia;4.Institute of Chemical and Environmental Engineering,Slovak University of Technology,Bratislava,Slovakia;5.Institute of Biotechnology and Food Science, Faculty of Chemical and Food Technology,Slovak University of Technology,Bratislava,Slovakia |
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| Abstract: | Biological molecules such as enzymes, cells, antibodies, lectins, peptide aptamers, and cellular components in an immobilized form are extensively used in biotechnology, in biorecognition and in many medicinal applications. This review provides a comprehensive summary of the developments in new immobilization materials, techniques, and their practical applications previously developed by the authors. A detailed overview of several immobilization materials and technologies is given here, including bead cellulose, encapsulation in ionotropic gels and polyelectrolyte complexes, and various immobilization protocols applied onto surfaces. In addition, the review summarises the screening and design of an immobilization protocol, practical applications of immobilized biocatalysts in the industrial production of metabolites, monitoring, and control of fermentation processes, preparation of electrochemical/optical biosensors and biofuel cells. |
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