Development and validation of an HPLC-DAD analytical procedure for quality control of damiana (Turnera diffusa), using an antioxidant marker isolated from the plant |
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Authors: | Pérez-Meseguer Jonathan Garza-Juárez Aurora Salazar-Aranda Ricardo Salazar-Cavazos Ma Luz de la Torre Rodríguez Yael C Rivas-Galindo Verónica Waksman de Torres Noemí |
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Institution: | Universidad Autónoma de Nuevo León, Faculty of Medicine, Analytical Chemistry Department, P.O. Box 2316, Sucursal Tecnológico, 64841, Monterrey Nuevo León, México. |
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Abstract: | Damiana is a shrub used as a dietary supplement and as an additive in several foods. This report presents the first validated analytical method for QC of products made from damiana. Through bioassay-guided fractionation, the compound showing the best antioxidant activity, determined using 2,2-diphenyl-1-picryl-hydrazyl assay in TLC, was purified and identified as 8-C-beta-6-deoxy-2-O-(alpha-1-rhamnopyranosyl)-xylo- hexopyranos-3-uloside] (Cl50 5.56 g/mL). This is the only natural source from which this compound has been reported, and has been found in all native damiana samples analyzed. Therefore, it was used as a biomarker to develop an HPLC-diode-array detection analytical procedure using the first-order derivative of the chromatograms. Separation was achieved using an AccQ Tag C18 150 x 3.9 mm (4 microm) column, with a gradient of methanol and 0.1% trifluoroacetic acid in water. The method was shown to be accurate, sensitive, and reproducible, providing a useful quantitative means to apply QC to damiana products. |
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