| 磁性壳聚糖微球用于酶的固定化研究：Ⅲ磁性壳聚糖微球的活化及其对脲酶的固化研究 |
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| 引用本文： | 任广智,李振华,等.磁性壳聚糖微球用于酶的固定化研究：Ⅲ磁性壳聚糖微球的活化及其对脲酶的固化研究[J].离子交换与吸附,2001,17(3):224-229. |
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| 作者姓名： | 任广智 李振华 |
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| 作者单位： | [1]吸附与分离功能高分子材料国家重点实验室，南开大学高分子化学研究所，天津300071 [2]吸附与分离功能高分子材料 |
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| 基金项目： | 教育部博士点基金(9800504) |
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| 摘 要： | 以环氧氯丙烷活化的磁性壳聚糖微球作为载体、对脲酶进行固定化研究，结果表明，在25℃时，活化磁性壳聚糖微球对脲酶的固定化在2h时就达到了最大值，固定化酶和自由酶的最适温度都在65℃左右，自由酶和固定化酶的米氏常数Km值分别为0．042mol/L和0．008mol/L，固定化酶的Km降低了5倍。
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| 关 键 词： | 固定化酶 脲酶 磁性壳聚糖微球 固定化 |
| 文章编号： | 1001-5493(2001)03-0224-06 |
| 修稿时间： | 2000年9月20日 |
THE IMMOBILIZATION OF
UREASE ON MAGNETIC CHITOSAN MICROSPHERES |
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| REN Guangzhi,LI Zhenhua,HE Binglin The State Key Laboratory of Functional Polymer Materials for Absorption and Sepa ration.THE IMMOBILIZATION OF
UREASE ON MAGNETIC CHITOSAN MICROSPHERES[J].Ion Exchange and Adsorption,2001,17(3):224-229. |
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| Authors: | REN Guangzhi LI Zhenhua HE Binglin The State Key Laboratory of Functional Polymer Materials for Absorption and Sepa ration |
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| Institution: | REN Guangzhi,LI Zhenhua,HE Binglin The State Key Laboratory of Functional Polymer Materials for Absorption and Sepa ration,Institute of Polymer Chemistry,Nankai University,Tianjin 300071 |
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| Abstract: | The magnetic chitosan microsphere were activated by epichlorohydrin in the presence of sodium hydroxide, which were used as carrier for the immobiliza tion of urease. It was found that the content of epoxy in the activated magnetic chitosan microsphere was 0.65mmol/g. The immobilizated urease will be obtained at 2.0h. The optimum temperature of the immobilizated urease was close to that o f the native urease. The half times for the decay of activity were about 2.0h fo r the immobilization urease and native urease. The optimum pH of the immobilized urease decreased by 0.4. The Km value of immobilized urease and native urease a re 0.008mol/l and 0.042mol/l, respectively. |
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| Keywords: | Immobilized enzyme Urease |
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