A novel competitive fluorescence immunoassay for the determination of dibutyl phthalate |
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| Authors: | Ming-Cui Zhang Qiong-E Wang Hui-Sheng Zhuang |
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| Institution: | (1) College of Environmental Science and Engineering, Donghua University, Shanghai, 201620, People’s Republic of China;(2) College of Chemistry and Chemical Engineering, Donghua University, Shanghai, 201620, People’s Republic of China;(3) College of Chemistry and Materials Science, Anhui Normal University, Wuhu, 241000, People’s Republic of China |
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| Abstract: | A novel, sensitive, and specific competitive fluorescence immunoassay has been developed for the quantitative determination
of dibutyl phthalate (DBP) using an antibody-coated plate format. Hapten was synthesized in order to produce polyclonal antibodies
against dibutyl phthalate. Polyclonal antisera to dibutyl phthalate were generated in rabbits and used to construct the fluorescence
immunoassay for measurement of dibutylphthalate. The assay had a detection limit of about 0.02 μg L−1, a dynamic range of approximately 0.1–300 μg L−1. Other similar phthalate compounds do not interfere significantly in the analysis using this immunoassay technique, and the
cross-reactivity rates were less than 10%. The study demonstrated that the developed antiserum and fluorescence immunoassay
procedure can be used to detect dibutyl phthalate in environmental samples such as tap water, river water, drinking water,
and leachate from plastic drinking water bottles. |
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| Keywords: | Dibutyl phthalate Determination Hapten Antibody Fluorescence immunoassay |
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