| 利用烟草高效转化功能基因体系的建立 |
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| 引用本文： | 邱立明,王艳,王瑜,代春英,马纪.利用烟草高效转化功能基因体系的建立[J].新疆大学学报(理工版),2008,25(1):23-27,35. |
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| 作者姓名： | 邱立明 王艳 王瑜 代春英 马纪 |
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| 作者单位： | 新疆生物资源基因工程重点实验室,新疆大学生命科学与技术学院,新疆乌鲁木齐830046 |
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| 基金项目： | 国家自然科学基金（30760056）及教育部春晖计划（2005020603）项目资助. |
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| 摘 要： | 对农杆菌介导的烟草遗传转化条件进行探索,以20 mg/L的潮霉素作为转化苗的筛选压,稀释至OD600为O.2～O.3的农杆菌对外植体浸染15 min,于添加300 mg/L头孢霉素的生根培养基中诱导生根,可稳定获得较高的阳性转化率.经PCR和PCR-Souttlern检测,阳性转基因植株达84.8%以上.通过本研究建立了一种稳定高效的功能基因烟草遗传转化体系.
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| 关 键 词： | 烟草 农杆菌 筛选压 浸染时间 生根培养基 利用 烟草 转化功能 基因体系 genes functional system genetic transformation tobacco efficient high 遗传转化体系 功能基因 稳定高效 研究 基因植株 检测 转化率 阳性 诱导生根 |
| 文章编号： | 1000-2839（2008）01-0023-05 |
| 收稿时间： | 2007-12-01 |
| 修稿时间： | 2007年12月1日 |
Establishment of high efficient tobacco genetic transformation system for functional genes |
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| QIU Li-ming,WANG Yan,WANG Yu,DAI Chun-ying,MA Ji.Establishment of high efficient tobacco genetic transformation system for functional genes[J].Journal of Xinjiang University(Science & Engineering),2008,25(1):23-27,35. |
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| Authors: | QIU Li-ming WANG Yan WANG Yu DAI Chun-ying MA Ji |
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| Abstract: | Conditions for tobacco Agrobacterium-mediated genetic transformation were explored. 20 mg / L of hygromycin were used as selection pressure to transformed seedlings, and explants were immerged into Agrobacterium with OD600 0. 2 - 0. 3 for 15 min, then the rooting culture contains 300 mg / L of cephalosporin was used for the induction of roots. Positive transgenic plants were 84. 8% by PCR and PCR-Southern blotting analysis, and an high efficient genetic transformation system was established. |
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| Keywords: | tobacco Agrohacterium selection pressure immerged time rooting culture medium |
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