| 二甲苯兰FF-溴化十六烷基三甲基铵-核酸共振光散射光谱研究及分析应用 |
| |
| 引用本文： | 欧阳振中,白珊,杜正华. 二甲苯兰FF-溴化十六烷基三甲基铵-核酸共振光散射光谱研究及分析应用[J]. 光谱实验室, 2010, 27(2): 645-647 |
| |
| 作者姓名： | 欧阳振中 白珊 杜正华 |
| |
| 作者单位： | 湖南长沙环境保护职业技术学院,长沙市雨花区井圭路10号,410004;海南师大附中,海口市美兰区灵山镇新大洲大道机场北路,571126 |
| |
| 摘 要： | 在pH9.90的Tris-HCl缓冲溶液中,阴离子染料二甲苯兰FF(XCFF)对阳离子表面活性剂溴化十六烷基三甲基铵(CTMAB)与核酸(fsDNA、ctDNA、yRNA)的共振光散射光谱有协同增强作用。考察了影响因素,在优化条件下研究了共振光散射强度与核酸浓度之间的关系,对于fsDNA、ctDNA、yRNA的线性范围分别为0.05—3.0mg/L、0.05—6.0mg/L、0.5—2.0mg/L,检出限分别为24.1、18.4、57.0μg/L。据此建立了一种测定核酸的新方法。
|
| 关 键 词： | 溴化十六烷基三甲基铵 二甲苯兰FF 核酸 共振光散射 |
Resonance Light Scattering Spectra of the Xylene Cyanal FF-Cetyltrimethyl Ammonium Bromide-Nucleic Acid System and Its Analytical Application |
| |
| Ouyang Zhen-Zhong,Bai Shan,Du Zheng-Hua. Resonance Light Scattering Spectra of the Xylene Cyanal FF-Cetyltrimethyl Ammonium Bromide-Nucleic Acid System and Its Analytical Application[J]. Chinese Journal of Spectroscopy Laboratory, 2010, 27(2): 645-647 |
| |
| Authors: | Ouyang Zhen-Zhong Bai Shan Du Zheng-Hua |
| |
| Affiliation: | Changsha Environmental Protection College;Changsha 410004;P.R.China;The Affiliated Middle School of Hainan University;Haikou 571126;P.R.China |
| |
| Abstract: | Resonance light-scattering(RLS) spectra of the xylene cyanal FF(XCFF)-cetyltrimethyl ammonium bromide(CTMAB)-nucleic acid system has been studied.In Tris-HCl buffer solution(pH=9.90),XCFF and nucleic acid react with CTMAB to form large particles of ion-association complex,which result in significant enhancement of RLS intensity.The spectral characteristics of RLS,the effective factors and optimum conditions of the reaction have been investigated.Under the optimum conditions,the enhanced RLS intensity is pro... |
| |
| Keywords: | Cetyltrimethyl Ammonium Bromide Xylene Cyanal FF Nucleic Acid Resonance Light Scattering |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
