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q value and parent energy optimization using a low-voltage ionization approach increases resolution in linear ion trap mass spectrometry |
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| Authors: | Simone Madama Ermelinda Falletta Amir Mohammad Malvandi Kevin Arzoni Carlo Brogna Marco Varelli Matteo Bertelli Matteo Conti Martina Larini Federico Guidugli Pietro Traldi Simone Cristoni |
| Affiliation: | 1. I.S.B. - Ion Source & Biotechnologies srl, Milan, Italy;2. University of Milan, Milan, Italy;3. Laboratory of Experimental Biochemistry and Molecular Biology, IRCCS Istituto Ortopedico Galeazzi, Milan, Italy;4. Craniomed, Avellino, Italy;5. Diagnostic Institute Varelli, Naples, Italy;6. MAGI'S LAB srl, Trento, Italy;7. Analytical Chemistry, Sant'Orsola Hospital, Bologna, Italy;8. Thermo Fisher Corporation, Milan, Italy;9. Institute of Paediatric Research Città della Speranza, Padova, Italy |
| Abstract: | In this work, the isolation step in the linear ion trap was performed using different “q values” conditions at a low collision-induced dissociation (CID) energy leading to the parent ion resolution improvements, reasonably due to better ion energy distribution. According to the results, we obtained a greater resolution and mass accuracy operating in both traditional electrospray and low voltage ionization near the q value = 0.778 and with a CID energy of 10%. This effect was evaluated with low-molecular-mass compounds (skatole and arginine). The proposed optimization yielded a superior instrument performance without adding technological complexity to mass spectrometry analyses. |
| Keywords: | ion trap low voltage ionization metabolites MS accuracy MS resolution q value |