Optimisation of chiral separation of omeprazole and one of its metabolites on immobilized α1-acid glycoprotein using chemometricsglycoprotein using chemometrics |
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Authors: | A Karlsson S Hermansson |
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Institution: | 1. Analytical Chemistry, Astra H?ssle AB, 431 83, M?lndal, Sweden
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Abstract: | Summary A strategy for the optimisation of direct chiral separation of omeprazole and a metabolite, hydroxi-omeprazole, in reversed
phase liquid chromatography is described.
A factorial design was used, where mobile phase pH, concentration of a mobile phase modifier, ionic strength and column temperature
were tested as the variables and enantioselective retention, column efficiency and asymmetry factor as the responses.
The experimental results were evaluated with multivariate analyses, which demonstrated that the column temperature and content
of mobile phase acetonitrile were by far the most important variables. The enantiomers of omeprazole and one of its metabolites
were baseline resolved within 15 minutes. The optimised chromatographic system was used for a separation of the enantiomers
of omeprazole and its main metabolite in a patient plasma sample. |
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Keywords: | Column liquid chromatography Experimental design Direct chiral resolution Omeprazole Hydroxy-omeprazole |
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