低氧亲和力血红蛋白类氧载体的制备及大鼠动脉血压响应

氧亲和力是血红蛋白氧载体非常重要的参数之一，但其高低还没有统一的认识，有一种理论认为低氧亲和力是造成高血压的原因。为了进一步研究氧亲和力与血压之间的关系，本文制备了两种低氧亲和力的血红蛋白氧载体。用高碘酸钠氧化棉子糖（高碘酸钠：棉籽糖=6：1，摩尔比），得到氧化均一的开环棉子糖；以其作为交联剂聚合脱氧猪血红蛋白，在聚合1 h得到了低氧亲和力（P50 = 43.1 torr）主要为分子内交联的64 kDa的血红蛋白，在聚合2 4 h得到了低氧亲和力（P50 = 51.5 torr）平均分子量为600 kDa的聚合血红蛋白；分别进行大鼠50%等容量换血实验，前者显著引起血压升高而后者血压平稳，证实高血压主要和64 kDa血红蛋白有关，低氧亲和力不是造成高血压的主要原因。

Preparation of Hemoglobin-based Oxygen Carrier with Low Oxygen Affinity and Arterial Blood Pressure Responses in Rat

Hemoglobin-based oxygen carriers(HBOCs) is a universally compatible,immediately available,oxygen-carrying resuscitative fluid being developed as a red cell substitute for use in urgent blood loss.The oxygen affinity is one of the most important parameters of HBOCs,and is not completely understood at present.It was reported that the low oxygen affinity is the main reason for vasoconstriction(hypertension) in transfusion.In order to study the relationship between the oxygen affinity and the hypertension,two d...