A novel electrochemical SPR biosensor |
| |
Institution: | 1. School of Bioresources and Technology, King Mongkut''s University of Technology Thonburi, Bangkhuntien Campus, Bangkok 10150, Thailand;2. Biochemical Engineering and Pilot Plant Research and Development Unit, National Center for Genetic Engineering and Biotechnology National Science and Technology Development Agency at King Mongkut''s University of Technology Thonburi, Bangkhuntien Campus, Pathum Thani 12120, Thailand |
| |
Abstract: | In this paper, we demonstrate for the first time that upon electrochemical oxidation/reduction, the transition in the conductivity of polyaniline (PAn) film on gold electrode surface leads to a large change of surface plasmon resonance (SPR) response due to a change in the imaginary part of dielectric constant of PAn film. Based on the amplifying response of SPR to the redox transformation of PAn film as a direct result of the enzymatic reaction between horseradish peroxidase (HRP) and PAn in the presence of H2O2, a novel PAn-mediated HRP sensor has been fabricated. The electrochemical SPR biosensor, unlike a usual binding assay with SPR, can afford a larger SPR response, and can also be reused by reducing the PAn film electrochemically to its reduced state. This method opens up a new route to the fabrication of SPR biosensor. |
| |
Keywords: | |
本文献已被 ScienceDirect 等数据库收录! |
|