Biocomposites of covalently linked glucose oxidase on carbon nanotubes for glucose biosensor |
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Authors: | Jian?Li Yue-Bo?Wang Jian-Ding?Qiu Da-cheng?Sun Email author" target="_blank">Xing-Hua?XiaEmail author |
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Institution: | (1) Key Laboratory of Analytical Chemistry for Life Science, Department of Chemistry, Nanjing University, Nanjing, 210093, PR China |
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Abstract: | The formation of covalently linked composites of multi–walled carbon nanotubes (MWCNT) and glucose oxidase (GOD) with high-function
density for use as a biosensing interface is described. The reaction intermediates and the final product were characterized
by using FT–IR spectroscopy, and the MWCNT-coated GOD nanocomposites were examined by atomic force microscopy (AFM) and transmission
electron microscopy (TEM). Interestingly, it was found that the GOD–MWCNT composites are highly water soluble. Electrochemical
characterization of the GOD–MWCNT composites that were modified on a glassy carbon electrode shows that the covalently linked
GOD retains its bioactivity and can specifically catalyze the oxidation of glucose. The oxidation current shows a linear dependence
on the glucose concentration in the solution in the range of 0.5–40 mM with a detection limit of 30 μM and a detection sensitivity
of 11.3 μA/mMcm2. The present method may provide a way to synthesize MWCNT related composites with other biomolecules and for the construction
of enzymatic reaction-based biofuel cells and biosensors.
Supported by grants from the National Natural Science Foundation of China (NSFC, No. 20125515; 90206037; 20375016) and the
Natural Science Foundation of Jiangsu Province (Grant No. BK 2004210) |
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Keywords: | Carbon nanotubes (CNT) Enzyme Biomaterials Glucose Biosensors |
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