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Biological functionalization and patterning of porous silicon prepared by Pt-assisted chemical etching |
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| Authors: | Hong-Fang Li Ya-Guang Wu |
| Affiliation: | a State Key Laboratory of Coordination Chemistry, School of Chemistry and Chemical Engineering, Nanjing National Laboratory of Microstructures, Nanjing University, Nanjing 210093, PR China b State Key Laboratory of Bioelectronics, Southeast University, Nanjing 210096, PR China |
| Abstract: | Porous silicon fabricated via Pt-assisted chemical etching of p-type Si (1 0 0) in 1:1:1 EtOH/HF/H2O2 solution possesses a longer durability in air and in aqueous media than anodized one, which is advantageous for biomedical applications. Its surface SiHx (x = 1 and 2) species can react with 10-undecylenic acid completely under microwave irradiation, and subsequent derivatizations of the end carboxylic acid result in affinity capture of proteins. We applied two approaches to produce protein microarrays: photolithography and spotting. The former provides a homogeneous microarray with a very low fluorescence background, while the latter presents an inhomogeneous microarray with a high noise background. |
| Keywords: | Porous silicon Protein microarray Surface chemistry Metal-assisted chemical etching NTA |
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