Computing Arithmetic Functions Using Immobilised Enzymatic Reaction Networks |
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Authors: | Nikita M Ivanov Mathieu G Baltussen Cristina Lía Fernández Regueiro Max T G M Derks Dr Prof Wilhelm T S Huck |
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Institution: | 1. Institute for Molecules and Materials, Radboud University, Heyendaalseweg 135, 6525AJ Nijmegen (The, Netherlands;2. Institute for Molecules and Materials, Radboud University, Heyendaalseweg 135, 6525AJ Nijmegen (The, Netherlands
co-first authors |
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Abstract: | Living systems use enzymatic reaction networks to process biochemical information and make decisions in response to external or internal stimuli. Herein, we present a modular and reusable platform for molecular information processing using enzymes immobilised in hydrogel beads and compartmentalised in a continuous stirred tank reactor. We demonstrate how this setup allows us to perform simple arithmetic operations, such as addition, subtraction and multiplication, using various concentrations of substrates or inhibitors as inputs and the production of a fluorescent molecule as the readout. |
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Keywords: | Analog Computing Enzymatic Reaction Networks Enzyme Immobilisation Molecular Computing Molecular Information Processing |
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