Investigation of Nutrient Feeding Strategies in a Countercurrent Mixed-Acid Multi-Staged Fermentation: Experimental Data |
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| Authors: | Aaron Douglas Smith Nur Ain Lockman Mark T Holtzapple |
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| Institution: | (1) Department of Chemical Engineering, Texas A&M University, 624 Jack Brown Building, College Station, TX 77843, USA;(2) Department of Chemical Engineering, Texas A&M University, College Station, TX 77843, USA;(3) Department of Chemical Engineering, Texas A&M University, 232 Jack Brown Building, College Station, TX 77843, USA |
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| Abstract: | Nutrients are essential for microbial growth and metabolism in mixed-culture acid fermentations. Understanding the influence
of nutrient feeding strategies on fermentation performance is necessary for optimization. For a four-bottle fermentation train,
five nutrient contacting patterns (single-point nutrient addition to fermentors F1, F2, F3, and F4 and multi-point parallel
addition) were investigated. Compared to the traditional nutrient contacting method (all nutrients fed to F1), the near-optimal
feeding strategies improved exit yield, culture yield, process yield, exit acetate-equivalent yield, conversion, and total
acid productivity by approximately 31%, 39%, 46%, 31%, 100%, and 19%, respectively. There was no statistical improvement in
total acid concentration. The traditional nutrient feeding strategy had the highest selectivity and acetate-equivalent selectivity.
Total acid productivity depends on carbon–nitrogen ratio. |
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