多巴胺改性海藻酸多孔支架的制备与性能

生物大分子海藻酸(Alg)由于其安全、无毒、可生物降解等特性而被广泛应用于组织工程领域。 受海洋贻贝蛋白结构的启发,多巴胺(DA)具有优异的粘附性能,在碱性水溶液条件下可发生氧化自聚形成聚多巴胺(PDA)。 以Alg为基体,加入PDA纳米粒子复合,并通过冷冻干燥法制备得到Alg/PDA多孔支架材料。 结果表明,Alg/PDA多孔支架材料具有较为规整的内部结构。 改变Alg质量浓度,Alg/PDA支架材料的孔径可控制在60~120 μm之间,孔隙率可控于80%~88%。 所得的支架材料具有适宜大小的孔径和孔隙率,结果表明支架材料对细胞无毒副作用。

Preparation and Properties of Polydopamine and Alginate Porous Complex Scaffolds

Alginate(Alg) is widely used in the field of tissue engineering because of its safe, non-toxic and biodegradable properties. Inspired by mussel adhesive protein, dopamine(DA) has excellent adhesion property and can self-oxidize to polydopamine(PDA) in weak basic buffer solution. Alg/PDA porous scaffolds were prepared by freeze-dry of Alg and PDA mixture. The results show that Alg/PDA porous scaffolds have relatively homogeneous internal structure. By changing the mass concentration of Alg, the pore size of the Alg/PDA complex scaffolds can be limited to 60~120 μm, and the porosity of the scaffolds can be controlled in the range of 80%~88% which is appropriate for the growth of bond cells. The biocompatibility results indicate that the scaffolds show low cytotoxicity.