荧光法测定糖果中的总糖含量

合成了带有荧光基团的分子探针,光谱显示探针对糖类物质具有较强的荧光响应.通过条件优化,选取328 nm作为荧光激发波长,p H 10.0的缓冲溶液为最佳测定介质,当测试溶液中葡萄糖浓度为0.05~1 mmol/L时,显示良好的响应线性.方法对果糖中总糖含量测定的检测限为6.2μmol/L,回收率在76.4%~90.0%,RSD达到3.18%.

Detection of Total Sugar in Candy by Fluorescence Spectrometry

In order to overcome the complicated operation and relatively low sensitivity of conventional method, a method to determine the total sugar of candy by fluorescence spectrometry has been established. The procedure started with the synthesis of a molecular probe bearing pyrene fluorephore, which shows strong fluorescent response towards carbohydrates. After optimization of the experiment, the determinaton of glucose concentraion was performed in a pH of 10. 0 aqueous buffer, with the assay solution being excited at 328 nm. The calibration curve showed good linearty over the glucose concentration of 0. 05~1 mmol/L. The limit of detection for the method herein reported was 6. 2μmol/L , with recovery in the range of 76. 4% ~90. 0%, and the RSD was 3. 18%.