Salt tolerance of desorption electrospray ionization (DESI) |
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Authors: | Ayanna U. Jackson Nari Talaty R. Graham Cooks Gary J. Van Berkel |
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Affiliation: | aDepartment of Chemistry, Purdue University and Bindley Bioscience Center, Discovery Park at Purdue University, West Lafayette, Indiana, USA bOrganic and Biological Mass Spectrometry Group, Oak Ridge National Laboratory, Oak Ridge, Tennessee, USA |
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Abstract: | The salt tolerance of desorption electrospray ionization (DESI) was systematically investigated by examining three different drug mixtures in the presence of 0, 0.2, 2, 5, 10, and 20% NaCl:KCl (1:1) from different surfaces. At physiological salt concentrations, the individual drugs in each mixture were observed in each experiment. Even at salt concentrations significantly above physiological levels, particular surfaces were effective in providing spectra that allowed the ready identification of the compounds of interest in low nanogram amounts. Salt adducts, which are observed even in the absence of added salt, could be eliminated by adding 0.1% 7 M ammonium acetate to the standard methanol:water (1:1) spray solvent. Comparison of the salt tolerance of DESI with that of electrospray ionization (ESI) demonstrated better signal/noise characteristics for DESI. The already high salt tolerance of DESI can be optimized further by appropriate choices of surface and spray solution. |
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