Development of xylose-fermenting yeasts for ethanol production at high acetic acid concentrations |
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Authors: | Mohandas Devaki V Whelan Douglas R Panchal Chandra J |
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Institution: | 1.Vetrogen Corporation, 1200 Wonderland Road South, Building 9, Unit 1, N6L 1A8, London, Ontario, Canada ; |
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Abstract: | Mutants resistant to comparatively high levels of acetic acid were isolated from the xylose-fermenting yeastsCandida shehatae andPichia stipitis by adapting these cultures to increasing concentrations of acetic acid grown in shake-flask cultures. These mutants were
tested for their ability to ferment xylose in presence of high acetic acid concentrations, in acid hydrolysates of wood, and
in hardwood spent sulfite liquor, and compared with their wild-type counterparts and between themselves. TheP. stipitis mutant exhibited faster fermentation times, better tolerance to acid hydrolysates, and tolerance to lower pH. |
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Keywords: | Index Entries" target="_blank">Index Entries Xylose fermentation acetic acid tolerance adaptation acid hydrolysates spent sulfite liquor |
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