Saccharide Sensing Using Gold and Silver Nanoparticles-A Review |
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Authors: | Kadir Aslan Jian Zhang Joseph R Lakowicz Chris D Geddes |
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Institution: | Center for Fluorescence Spectroscopy, University of Maryland Biotechnology Institute, 725 West Lombard Street, Baltimore, Maryland 21201, USA. |
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Abstract: | We review new methodologies for glucose sensing from our laboratories based on the specific biological interactions between Con A, dextran-coated gold nanoparticles and glucose, and the interactions between dextran, glucose, and boronic-acid capped silver nanoparticles in solution. Our new approaches promise new tunable glucose sensing platforms. Dextran-coated gold nanoparticles were aggregated with the addition of Con A resulting in increase an in absorbance of nanoparticles at 650 nm, where the post-addition of glucose caused the dissociation of the aggregates and thus a decrease in the absorbance at 650 nm. The interaction of glucose and dextran with boronic acid-capped silver nanoparticles in solution resulted in enhanced luminescence intensity cumulatively due to surface-enhanced fluorescence and the decrease in absorbance at 400 nm, with an increase in absorbance at 640 nm. Lifetime measurements were used to distinguish the contribution from the surface-enhanced fluorescence. TEM was employed to assess the aggregation of nanoparticles. |
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Keywords: | Gold colloids silver colloids glucose sensing nanosensors monosaccharide polysaccharide plasmons surface plasmon resonance |
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