北五味子的液相色谱指纹图谱的建立

利用反相高效液相色谱法建立中药材北五味子的指纹图谱,为科学评价及有效控制北五味子质量提供了新方法。实验分析了10个不同产地的北五味子样品,采用国家食品药品监督管理局推荐的“中药色谱指纹图谱相似度评价系统(2004 A版)”计算处理,建立了由26个指纹峰和19个共有峰组成的北五味子指纹图谱,确定了5个主要的指纹峰。通过夹角余弦法和相关系数法计算了北五味子10个样本与指纹图谱间的相似度,得到了满意的结果。所建立的指纹图谱可以用来区别不同产地北五味子药材的优劣,为进一步控制北五味子的质量提供依据。

Establishment of high performance liquid chromatographic fingerprint of Fructus schisandrae chinensis

The chromatographic fingerprint was established for evaluating and controlling the quality of Fructus schisandrae chinensis by reversed-phase high performance liquid chromatography (RP-HPLC). Ten different original samples were analyzed, and the chromatographic fingerprint of Fructus schisandrae chinensis was constructed with 26 fingerprint peaks, among which 19 peaks were common fingerprint peaks and 5 main compounds were identified using the Similarity Evaluation System of Chromatographic Fingerprint of Traditional Chinese Medicine (Version 2004 A) recommended by State Food & Drug Administration. Methods of vector cosine and correlation coefficient were used to calculate the similarity of ten samples, and satisfactory results were obtained. The chromatographic fingerprint can be used to differentiate the raw materials from different sources and be served as a powerful tool for the further quality control of Fructus schisandrae chinensis.