| 活体动物代谢过程的31P NMR研究——方法与初步结果 |
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| 引用本文： | 颜贤忠,朱好勤.活体动物代谢过程的31P NMR研究——方法与初步结果[J].波谱学杂志,1994,11(3):257-264. |
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| 作者姓名： | 颜贤忠 朱好勤 |
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| 作者单位： | 1. 北京军事医学科学院仪器测试中心, 北京 100850;2. 北京毒物药物研究所, 北京 100850;3. 中国药科大学物理教研室, 南京 210009 |
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| 摘 要： | 利用表面线圈探头测得了小鼠大脑,大腿肌肉和肝脏的体内31P NMR谱图。观察到PCr,ATP,PME,PDE,Pi等化合物的共振峰,并确定了各谱峰的化学位移。由大脑的Pi化学位移计算得到其细胞内pH值为7.15.用差谱方法解决了因定位不准而造成的肝脏谱图不纯。观察了小鼠大脑在缺氧状态下的代谢变化过程。当O2浓度降至2%时,PCr开始下降,降到1%时,Pi明显升高。从空气变化到1%的O2含量,致使小鼠死亡,小鼠大脑的pH值从7.16降到6.46。
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| 关 键 词： | In vivo NMR 代谢 大脑缺氧 |
| 收稿时间： | 1993-05-31 |
IN VIVO 31P NMR STUDIES OF METABOLISM IN ANIMALS——Methods and Preliminary Results |
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| Yan Xianzhong,Li Hua,Feng Rui,Du Zehan,Ruan Jinxiu,Zhu Haoqin.IN VIVO 31P NMR STUDIES OF METABOLISM IN ANIMALS——Methods and Preliminary Results[J].Chinese Journal of Magnetic Resonance,1994,11(3):257-264. |
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| Authors: | Yan Xianzhong Li Hua Feng Rui Du Zehan Ruan Jinxiu Zhu Haoqin |
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| Institution: | 1. Academy of Military Medical Science, Beijing 100850;2. China Pharmaeeutical University, Nanjing 210009 |
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| Abstract: | Metabolism of mice was studied by in vivo 31P NMR spectroscopy.spectra of brain,leg muscle and liver were obtained, using a two-turn surface coil.Peaks of PCr, ATP,PME PDE and Pi were observed and their Chemical shifts determined,The pH value of brain is 7.15, according to the chemical shift of Pi.The contaminated spectrum of liver was corrected by subtraction of a muscle spectrum from the observed spectrum.Metabolism of mice brain in hypoxia was studied.The pH value of brain decreased from 7.1 6 to 6.46 when mice died at the end of experiment. |
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| Keywords: | in vivo NMR Metabolism Brain hypoxia |
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