高效液相色谱法测定糕点中的柠檬黄、日落黄

建立高效液相色谱法测定糕点中的柠檬黄、日落黄的方法。样品经水浸泡提取,于涡旋混匀器中快速混匀,浸泡放置过夜,取离心后的上清液,以甲醇和乙酸铵溶液梯度淋洗,用紫外检测器检测,柠檬黄、日落黄分别采用430 nm和482 nm检测波长。线性相关系数为0.999,柠檬黄、日落黄的检出限分别为0.4,0.3 mg/kg。样品加标回收率为72.5%~82.0%,柠檬黄、日落黄测定结果的相对标准偏差分别为4.8%和3.7%(n=5)。方法简便快捷,节省试剂,减少了杂质干扰,提高了灵敏度。

Determination of Lemon Yellow and Sunset Yellow in Pastry by High Performance Liquid Chromatography

The method for determination of lemon yellow and sunset yellow in pastry by high performance liquid chromatography was established. Samples was extracted by water immersion, and mixed rapidly by vortex mixer, and than soaked overnight. After taking centrifugal supernatant, lemon yellow and sunset yellow were determined with gradient elution of methanol and ammonium acetate solution by UV detector at 430 nm and 482 nm, respectively. The linear correlation coefficient was 0.999, and the detection limit of lemon yellow and sunset yellow was 0.4 and 0.3 mg/kg, respectively. The recovery was 72.5%-82.0%,and the relative standard deviation of determination results was 4.8%and 3.7%（n=5） for lemon yellow and sunset yellow. This method is simple and quick approach with less reagent,reducing the impurity interference and increasing the sensitivity at the same time.