β-环糊精与阿霉素相互作用荧光光谱

用荧光光谱技术确定了β-环糊精（β-CD）与阿霉素（ADM）之间的超分子包络物ADM-β-CD的形成。 研究了时间、浓度和温度对包络反应的影响，确定了包络物形成的化学计量比，计算了在不同温度下的包络常数和包络反应的热力学参数。 在33 ℃、pH=7.0时，包络常数K=2.98×106 L/mol，且包络常数随温度升高而变小，该包络反应属于焓驱动的自发的放热反应。 由于β-CD对ADM的天然荧光有增强作用，因此，ADM与β-CD形成超分子包络物后，荧光强度增大，荧光光谱是研究ADM与β-CD超分子包络反应的很好的技术。 求得β-CD增敏荧光法定量测定ADM的线性回归方程为:F=5.64×108c+47.26，相关系数r=0.9985，检测下限为6.30×10-8 mol/L。

Fluorescence Spectroscopic Investigation of the Interaction between 6-Cyclodextrin and Adriamycin

The fluorescence spectroscopic technique has been used to demonstrate the formation of the inclusion complex of adriamycin(ADM) and β-cyclodextrin(β-CD). The effects of the reaction time, concentration and temperature on the inclusion reaction are investigated. The chemical stoichiometric ratio for the formation of the inclusion complexes is determined. The inclusion constant and thermodynamic parameters of the inclusion reactions at different temperatures are calculated. At 33 ℃ and pH=7.0， the inclusion constant, K is 2.98×106 L/mol. The K value diminishes with the increase of temperature. Thus, the inclusion reaction is an enthalpy driving and spontaneous exothermic reaction. After ADM and β-CD form the inclusion complex, the fluorescence intensity is increased because β-CD can increase the natural fluorescence of ADM. Therefore, the fluorescence spectroscopic technique is an excellent technique for the investigation of the inclusion reaction of ADM and β-CD. The linear regression equation of the quantitative determination of ADM by β-CD sensitized fluorescence method is F=5.64×108c+47.26, with a correlation coefficient of 0.9985. The detection limit is 6.30×10-8 mol/L.