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Production of α-keto acids Part I. Immobilized cells ofTrigonopsis variabilis containing D-amino acid oxidase |
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| Authors: | Peter Brodelius Kjell Nilsson Klaus Mosbach |
| Institution: | (1) Pure and Applied Biochemistry, University of Lund, Chemical Center, PO Box 740, S-22007 Lund, Sweden |
| Abstract: | Whole cells ofTrigonopsis variabilis were immobilized by entrapment in Ca2+-alginate and used for the production of α-keto acids from the corresponding D-amino acids. The D-amino acid oxidase within the immobilized cells has a broad substrate specificity. Hydrogen peroxide formed in the enzymatic reaction was efficiently hydrolyzed by manganese oxide co-immobilized with the cells. The amino acid oxidase activity was assayed with a new method based on reversed-phase HPLC. Oxygen requirements, bead size, concentration of cells in the beads, flow rate, and other factors were investigated in a “ trickle-bed ” reactor. |
| Keywords: | Production of α -keto acids α -keto acids production of immobilized cells in the production of α -keto acids Trigonopsis variabilis in the production of α -keto acids D-amino acid oxidase in α -keto acid production oxidase D-aminoacid in α -keto acid production manganese oxide co-immobilized with whole cells liquid chromatography assay of D-amino acid oxidase activity chromatography liquid of D-amino acid oxidase activity |
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