Discussion of the protein characterization techniques used in the identification of membrane protein targets corresponding to tumor cell aptamers |
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Authors: | Zhukang Guo Yuan Liu Nongyue He Yan Deng Lian Jin |
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Institution: | State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, China; Hunan Key Laboratory of Biomedical Nanomaterials and Devices, Hunan University of Technology, Zhuzhou 412007, China |
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Abstract: | Aptamer is an oligonucleotide chain with specific binding ability to protein and other targets, which is widely used in many fields. Because of its ability to screen the premise of unknown targets, it can be used to discover some novel tumor markers, i.e., membrane proteins that are specifically highly expressed on the surface of tumor cells. Tumor markers can be used in many fields such as early diagnosis and treatment, and a new type of tumor marker proved to be effective can significantly improve the therapeutic effect of such tumors. However, further characterization of newly acquired membrane proteins is essential for their clinical use as tumor markers. This review first briefly introduced the process of obtaining novel tumor markers from nucleic acid aptamers. Next, the commonly used protein characterization methods could be used as a technical means to identify membrane protein targets corresponding to tumor cell aptamers, to clarify the principles, advantages and disadvantages of various means, and to analyze the most suitable situations for various experimental methods. Finally, the outlook was made and the characterization methods that should be used in such experiments were summarized. |
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Keywords: | Aptamer Cell-SELEX Tumor Membrane protein Tumor marker |
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