SIZE EXCLUSION CHROMATOGRAPHIC COLUMN PACKED WITH REGENERATED CELLULOSE GELS*

 Microporous regenerated cellulose gel particles were prepared by mixing cellulose cuoxam with silk fibroin aspore former, and the mean pore size and pore volume of the pallicles were 525 nm and 7.27 mL g^-1, respectively. Apreparative size-exclusion chromatography (SEC) column (550 mm×20 mm) packed with the cellulose gel particles wasused for the fractionation of two polysaccharides Dextran 07 (M_w = 7.14×10⁴, d= 1.7) and Dextran 50(M_w = 50.5×10⁴,d = 3.8) in water phase. The fractionation range of the stationary phase covered M_w from 3×10³ to 1.1×10⁶.The daily throughput was 2.9 g for Dextran 07(D07) and 4.3 g for Dextran 50(D50) with a flow-rate of 1.5 mL min^-1.The fractions obtained by using the SEC were analyzed by an analytical SEC combined with laser light scattering(LLS),and the polydispersity indices of fractions for Dextran 07 and Dextran 50 were determined to be 1.34-1.57 and 4.53-3.36,respectively.The preparative SEC is a simple,rapid,and suitable means not only for the fractionation of polysaccharides in water but also for other polymers in organic solvents.